Fig 1: RAP1 and GFAP? colocalize with subunits of the ?-secretase complex. SH-SY5Y cells were grown, fixed, and probed with antibodies as indicated in each panel. Representative images are shown that include images of each fluorophore individually with a larger, merged image. The box in the merged images indicates the region that is increased in size for the inset at the top right corner. Arrows in the inset indicate examples of colocalization. a) RAP1 and GFAP? colocalize. Cells were probed with DAPI, antibodies to RAP1 and antibodies to GFAP?. b) RAP1 colocalizes with the ?-secretase subunit PSENEN. Cells were probed with DAPI, antibodies to RAP1, and antibodies to PSENEN. c) RAP1 and GFAP? colocalize with the ?-secretase subunit PS1. DAPI stain was not used in this image. Cells were probed with antibodies to PS1, antibodies to RAP1 and antibodies to GFAP?.